Real-time quantification of RNA polymerase activity using a “broken beacon”
نویسندگان
چکیده
منابع مشابه
Quantification and Optimization of Candida albicans DNA in Blood Samples Using Real- Time PCR
Background: Candida albicans (C. albicans) is a major cause of candidaemia in people with impaired immunity. Blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. We established a real-time PCR assay for C. albicans detection in blood by LightCycler PCR and melting curve analysis. Methods: Five milliliter blood samples from...
متن کاملA molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time.
The design of new assay formats that allow fast parallel screening of enzyme function plays a pivotal role in the discovery of new products and reagents ranging from industrial processes to diagnosticsJ'] Furthermore, drug discovery and protein engineering are increasingly performed by combinatorial approaches, in which progress is tightly linked to the development of suitable screening systems...
متن کاملDetection and quantification in real-time polymerase chain reaction
The estimation of the concentration of an infectious agent in the environment is a key step to trigger an alert when there is a biological threat. This concentration can be obtained trough a quantitative polymerase chain reaction (qPCR). Nevertheless, standard real-time procedure do not address detection delay which is a main concern in alert triggering. Therefore, we propose a method based on ...
متن کاملBayesian estimation for quantification by real-time Polymerase Chain Reaction
The aim of the Quantitative Polymerase Chain Reaction is to determine the initial amount X0 of specific nucleic acids from an observed trajectory of the amplification process, the amplification being achieved through successive replication cycles. This process depends on the efficiency {pn}n of replication of the molecules, pn being the probability that a molecule will duplicate at replication ...
متن کاملQuantification Strategies in Real-time Polymerase Chain Reaction
The present chapter describes the quantification strategies used in real-time RT-PCR (RT-qPCR), focusing on the main elements that are essential to fulfil the MIQE guidelines. The necessity of initial proper data adjustment and background correction is discussed to allow reliable quantification. The advantages and disadvantages of the absolute and relative quantification approaches are also des...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2007
ISSN: 0003-2697
DOI: 10.1016/j.ab.2006.12.012